(HOME) Subject: endometriosis/infertility/ROS

   
   Fertil Steril 1997 Nov;68(5):826-30
   
Importance of reactive oxygen species in the peritoneal fluid of women with
endometriosis or idiopathic infertility.

    Wang Y, Sharma RK, Falcone T, Goldberg J, Agarwal A
    
   Department of Urology, Cleveland Clinic Foundation, Ohio 44195, USA.
   
   OBJECTIVE: To determine whether reactive oxygen species in peritoneal
   fluid might be a factor in infertility. DESIGN: Prospective study.
   SETTING: Andrology laboratory and gynecology clinic at a tertiary care
   facility. PATIENT(S): Women with endometriosis (n = 15) or idiopathic
   infertility (n = 11) who underwent laparoscopy for infertility.
   Patients undergoing tubal ligation served as controls (n = 13).
   INTERVENTION(S): Aspiration of peritoneal fluid. MAIN OUTCOME
   MEASURE(S): Reactive oxygen species levels, presence of
   polymorphonuclear granulocytes, and leukocyte distribution in
   peritoneal fluid. RESULT(S): Reactive oxygen species were present in
   the peritoneal fluid of patients with endometriosis, idiopathic
   infertility, and tubal ligation. Levels of reactive oxygen species did
   not show a statistically significant difference between patients with
   endometriosis and the control group in either unprocessed or processed
   (cell-free) peritoneal fluid, but did differ significantly between
   patients with idiopathic infertility and controls in processed
   peritoneal fluid. Polymorphonuclear granulocytes (> 1 x 10(6)/mL) were
   not present in the peritoneal fluid of any patient. Macrophage
   concentrations of peritoneal fluid did not differ significantly
   between controls and patients with endometriosis or idiopathic
   infertility. CONCLUSION(S): Reactive oxygen species in the peritoneal
   fluid may not affect fertility directly in women with endometriosis;
   however, they may have a role in patients with idiopathic infertility.
   
   PMID: 9389810, UI: 98051249
     _________________________________________________________________
   
   Save the above report in [Macintosh] [Text] format
   Order documents on this page through Loansome Doc
     _________________________________________________________________


Subject: fertility/iron

   
   J Toxicol Environ Health A 2001 Feb 23;62(4):259-67
   
In vitro effects of metal ions (Fe2+, Mn2+, Pb2+) on sperm motility and lipid
peroxidation in human semen.

    Huang YL, Tseng WC, Lin TH
    
   School of Technology for Medical Sciences, Kaohsiung Medical
   University, Taiwan, Republic of China. yelihu@cc.kmu.edu.tw
   
   [Medline record in process]
   
   The effects of divalent manganese ion (Mn2+), ferrous iron (Fe2+), and
   lead ion (Pb2+) on human sperm motility and lipid peroxidation were
   examined. Human semen from healthy male volunteers was incubated with
   0, 5, 50, or 500 ppm divalent metal ions, and the sperm motility was
   determined at 0, 2, 4, 6, or 8 h by microscopy. Malondialdehyde (MDA)
   levels in seminal plasma was measured by high-performance liquid
   chromatography after 8 h of exposure. The results showed that 500 ppm
   Mn2+ or Pb2+ significantly inhibited sperm motility without an
   accompanying change in seminal MDA levels. Incubation with Fe2+
   significantly inhibited sperm motility at 5 ppm, associated with a
   marked rise in MDA levels. Our results suggested that Fe2+ may induce
   lipid peroxidation to inhibit sperm motility. In the case of Mn2+ and
   Pb2+ there is an absence of seminal lipid peroxidation and the
   observed inhibition of sperm motility at high concentrations is not
   biologically or environmentally relevant.
   
   PMID: 11245395, UI: 21139014
     _________________________________________________________________
   
 

(HOME)