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Subject: endometriosis
Gynecol Obstet Invest 1998;46(1):58-60
Chemical assay of iron in ovarian cysts: a new diagnostic method to evaluate
endometriotic cysts.
Iizuka M, Igarashi M, Abe Y, Ibuki Y, Koyasu Y, Ikuma K
Department of Obstetrics and Gynecology, Gunma University School of
Medicine, Maebashi, Japan.
CA-125 is abundantly secreted from ovarian endometriotic cysts, but is
not specific to endometriosis. In order to develop a new, more
specific diagnostic marker for endometriosis, the iron concentrations
in various ovarian cysts were assayed. The ovarian cysts were
punctured and the contents aspirated laparoscopically,
laparotomically, or transvaginally. The iron concentration in the
ovarian cystic fluid was assayed using a spectrophotometer after
protein precipitations and chromogen treatment. The iron
concentrations in ovarian cysts were 69.5+/-10.4 micromol/l in serous
cystadenomas, 73.5+/-29.3 in mucinous cystadenomas, 65.4+/-12.4 in
dermoid cysts, and 92.5+/-18.2 micromol/l in adenocarcinomas. On the
other hand, high iron concentrations were demonstrated in
endometriotic cysts (1,749.6+/-41.5 micromol/l), a lutein cyst
(1,393.8), hemorrhagic corpus luteum (1,957.5) and endometrioid
adenocarcinomas (1,860.9+/-157.9 micromol/l). Cytological smear tests
of the contents as well as histological examination allowed
differential diagnosis between endometriosis and endometrioid
adenocarcinoma. In conclusion, assay of the ovarian cystic iron
concentration is a useful diagnostic tool for the evaluation of
ovarian endometriotic cysts.
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Subject: endometriosis
Ultrastruct Pathol 1997 May-Jun;21(3):273-80
The lipofuscin-iron association in pigmentosis tubae.
Munichor M, Kerner H, Cohen H, Iancu TC
Department of Pathology, Rambam Medical Center, Haifa, Israel.
Pigmentosis tubae (PT) is a rare condition characterized by the
presence of numerous lipofuscin-laden macrophages in the lamina
propria of the fallopian tube. Two women, who also had endometriotic
ovarian cysts, showed polypoid pigmented tubal mucosae. In addition to
lipofuscin, occasional cells showed spotty positivity for iron.
Ultrastructural examination of the tubal mucosa showed the
lipofuscin-containing bodies, which were similar to
lipofuscin-containing lysosomes found in other pigmented conditions.
Cytoplasmatic ferritin and hemosiderin in siderosomes were observed in
macrophages and endothelial cells of the lamina propria. The present
study is the first to demonstrate the presence of iron-containing
particles and lipofuscin in the residual bodies of PT. The origin of
the excess iron is not clear, but erythrophagocytosis and an abnormal
tubal environment could play a role. Iron-promoted lipid peroxidation
may alter the lysosomal membranes and contribute to the excessive
accumulation of lipofuscin in these cells.
Comments:
* Comment in: Ultrastruct Pathol 1997 Nov-Dec;21(6):605
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Subject: endometriosis
Fertil Steril 1995 Jul;64(1):62-4
De novo formation of adhesions in endometriosis: the role of iron and free
radical reactions.
Arumugam K, Yip YC
Department of Obstetrics and Gynaecology, University of Malaya, Kuala
Lumpur, Malaysia.
OBJECTIVE: To show that raised iron levels in the peritoneal fluid
(PF) of patients with endometriosis catalyze free radical reactions
that results in the tissue destruction and fibrosis seen in these
patients. DESIGN: A case-controlled study of the iron levels
(microgram/mL) in the pelvic PF of 12 patients with moderate-to-severe
disease, 15 patients with minimal-to-mild disease and in 17 women with
normal pelvises were compared. As an index of free radical reactions
through lipid peroxidation, the levels of malondialdehyde levels
(ng/mL) were assessed simultaneously in the same specimens. RESULTS:
Controlling for the phase of the menstrual cycle, significantly higher
levels of iron were seen in patients with endometriosis, the levels
being correlated with the severity of the disease. However no such
corresponding relationship was seen in the malondialdehyde levels in
the PF. CONCLUSIONS: These results suggest that raised iron levels in
the PF do not play a role in catalyzing free radical reactions as
judged by the degree of lipid peroxidation.
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Subject: fibroid/ferritin
Chung Hua Fu Chan Ko Tsa Chih 1991 Jan;26(1):21-3, 61
[Evaluation of serum ferritin in female genital neoplasms].
[Article in Chinese]
Dong MY
Obstetric and Gynecologic Hospital, Zhejiang Medical University,
Hangzhou.
Serum ferritin concentrations of 50 normal women and 90 patients with
neoplasms of female genital tract were determined by radioimmunoassay.
The mean value of serum ferritin in 23 cases of ovarian carcinoma was
402.04 micrograms/L, significantly higher than that of normal subjects
and patients with benign genital neoplasms. Serum ferritin levels in
patients with endometrial carcinoma, endometrial stromal sarcoma, and
benign genital neoplasms were significantly higher than that of the
normal subjects. There was a positive correlation between the serum
ferritin level and the clinical stage of ovarian carcinoma. The serum
ferritin determination is useful in the diagnosis, differential
diagnosis and prognosis of ovarian cancers.
PMID: 1848498, UI: 91168663
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